ZFIN Figure: Pollock et al., 2021, Figure 4.

ZFIN ID: ZDB-FIG-210708-72

ZFIN ID: ZDB-FIG-210708-72

Pollock et al., 2021 - Transcriptomic analysis of zebrafish prion protein mutants supports conserved cross-species function of the cellular prion protein. Prion 15:70-81 Full text @ Prion

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibody:	Ab2-cdh1
Fish:	AB AB + MO1-prnpb prnpb^{ua5003/ua5003}; prnprs3^{ua5001/ua5001}
Knockdown Reagent:	MO1-prnpb
Anatomical Term:	DEL plasma membrane
Stage:	Shield

PHENOTYPE:

Fish:	AB + MO1-prnpb prnpb^{ua5003/ua5003}; prnprs3^{ua5001/ua5001}
Knockdown Reagent:	MO1-prnpb
Observed In:	protein localization whole organism
Stage Range:	Shield to Protruding-mouth

Figure 4.

(A–C) There does not appear to be a difference in the maturation or localization of e-cadherin in either 5ng prp1 morpholino injected AB zebrafish or uninjected prp1^{ua5003/ua5003}; prp2^{ua5001/ua5001} homozygous mutant fish compared to uninjected wild-type controls. (F–E) After 3dpf wild-type larvae injected with prp1 MO (f) show significant signs of necrosis and developmental abnormalities compared to uninjected and control injected larvae (d, e)

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab2-cdh1	IHC	AB	standard conditions	Shield	DEL plasma membrane
	IHC	AB + MO1-prnpb	standard conditions	Shield	DEL plasma membrane
	IHC	prnpb^{ua5003/ua5003}; prnprs3^{ua5001/ua5001}	standard conditions	Shield	DEL plasma membrane

Phenotype Details

Fish	Conditions	Stage	Phenotype
AB + MO1-prnpb	standard conditions	Shield	protein localization process quality, normal
		Protruding-mouth	whole organism morphology, abnormal
		Protruding-mouth	whole organism necrotic, abnormal
prnpb^{ua5003/ua5003}; prnprs3^{ua5001/ua5001}	standard conditions	Shield	protein localization process quality, normal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Prion