FIGURE

Figure 2.

ID
ZDB-FIG-210708-68
Publication
Pollock et al., 2021 - Transcriptomic analysis of zebrafish prion protein mutants supports conserved cross-species function of the cellular prion protein
Other Figures
All Figure Page
Back to All Figure Page
Figure 2.

RNA-Sequencing show 1249 genes with an increase or decrease of log2 fold change of 0.5 between wild-type and compound homozygous prion mutant zebrafish larvae. (A) Scatter graph showing relative FPKM values for wild-type (X-axis) and mutant (Y-axis) genes after RNA-sequencing. (B) Methodology diagram showing RNA-sequencing workflow. Two groups, wild-type and prion mutant (prp1ua5003/ua5003; prp2ua5001/ua5001) homozygous fish, each with three replicates containing a pool of 50 3dpf larvae were processed and sent for RNA-sequencing. Heatmap displays sample of the 25 genes with a biggest differential abundance in FPKM in wild-types compared to mutants. (C) Relative transcript abundance between wild-type and prion mutant fish comparing prp1 and prp2 through both RNA-sequencing and RT-qPCR analysis. D) Relative transcript abundance of ncam1a and st8sia2 through both RNA-sequencing and RT-qPCR analysis. * = P < 0.05
Expression Data
Genes:
Fish:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Prion
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.