Fig. 7

AThe HMGB3 levels of CNE2-NC-exo and CNE2-shHMGB3-exo were measured by western blot. B Flow cytometry analysis showed the percentage of gDNA and HMGB3 expression in exosomes of CNE2 cell supernatant. C The CCK8 assays were performed to measure proliferation of HUVECs treated with the two exosomes. D The tube formation assays were performed to measure tube forming ability of HUVECs treated with various exosomes. The scale bar represents 200 μm. E Gross-observation exosomes modulated angiogenesis. F Frozen slices of the Matrigel plugs were stained with eosin-hematoxylin. The scale bar in 200× images represents 100 µm. The scale bar in 400× images represents 50 µm. G The GFP‐positive (green) intersegmental vessel (ISV) and CNE2 cells (red) are photographed by a microscope for 30 embryos per group. The red box was further photographed with a confocal microscope. The red arrow indicates a vascular defect. White arrows indicate metastasis nodes. H Percentage of vascular formation of ten embryos per group. I Quantification of migratory cell numbers. All experiments were conducted with three independent replicates. Mean ± SD, **P < 0.05, **P < 0.01, ***P < 0.001, student’s test.