FIGURE

Fig. 3

ID

ZDB-FIG-210612-3

Publication

Zhang et al., 2021 - Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis

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Fig. 3

HMGB3 knockdown reduces NPC cell proliferation and migration in vitro.

A HMGB3 protein levels in NP69 and NPC cell lines (CNE1, CNE2, 5–8 F and 6–10B) were determined by western blot. B HMGB3 mRNA levels in NP69 and NPC cell lines were examined by qRT-PCR (one-way ANOVA). C Transfection efficiency of three NPC lines (CNE1, CNE2 and 5–8 F) was measured by western blot. CON control, LIP lip2000, NC negative control, SH1 shHMGB3-1, SH2 shHMGB3-2, SH3 shHMGB3-3. D The transwell assay was performed to measure cell migration. The scale bar represents 50 µm. E Statistical comparison of the cell number of the transwell assay in the two groups. F The wound closure assay was performed to measure cell migration. The scale bar represents 200 µm. G Statistical comparison of the percentage of wound width in the two groups. H The CCK8 assays were performed to measure cell proliferation. All experiments were conducted with three independent replicates. I The levels of HMGB3 and EMT markers in NPC cell lines after knockdown or overexpression of HMGB3 were determined by western blotting. GAPDH expression was used as a loading control. Mean ± SD, **P < 0.01, ***P < 0.001,****P < 0.0001, student’s test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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