Fig. 3
- ID
- ZDB-FIG-210519-89
- Publication
- Tseng et al., 2021 - The role of Niemann-Pick type C2 in zebrafish embryonic development
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(A) Live 3 dpf npc2+/+ and npc2m/m larvae were stained with LysoTracker Red (LysoR) for 1 h and destained in egg water for 2 h before images were taken. npc2m/m larva showed intense LysoTracker Red staining of the olfactory placodes (arrowheads). A total of 47 npc2+/+ and 59 npc2m/m larvae were examined in three experiments. All examined larvae displayed the same staining patterns as shown here. Scale bars: 100 μm (individual); 400 μm (overlay). (B) Live double staining of npc2+/+ and npc2m/m larvae with YO-PRO-1 (neuromast cell marker) and LysoTracker Red. npc2m/m larva exhibited intense LysoTracker Red staining along lateral line neuromasts but the overall pattern of neuromast organization was unaffected. A total of 67 npc2+/+ and 75 npc2m/m larvae were examined from three experiments. All larvae displayed the same staining. Scale bars: 1 mm. (C) Filipin-positive puncta were present in 5 and 7 dpf npc2m/m larvae, indicating unesterified cholesterol accumulation. A total of 26 npc2+/+ and 19 npc2m/m 5 dpf larvae and 23 npc2+/+ and 28 npc2m/m 7 dpf larvae were examined in three experiments. All examined larvae displayed the same staining patterns. Scale bars: 200 μm. |
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Observed In: | |
Stage Range: | Protruding-mouth to Days 7-13 |