FIGURE

Figure 2.

ID

ZDB-FIG-210518-71

Publication

Cechmanek et al., 2021 - Retinal Pigment Epithelium and Neural Retinal Progenitors Interact Via Semaphorin 6D to Facilitate Optic Cup Morphogenesis

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Figure 2.

sema6d mutants do not display gross morphologic defects. A, Schematic of the two sema6d mutant alleles; sema6d^ca302 mutants contain a five-base pair deletion and two-base pair mismatch in exon6, and sema6d^ca303 mutants contain a 16-base pair insertion in exon10, both predicted to generate proteins that truncate prematurely within the SEMA domain and result in the loss of the transmembrane domain (TMD) and the intracellular domain (ICD). B–E, Lateral views of whole-mount embryos processed for bhlhe40 ISH, revealing normal gross embryonic morphology and somite labeling in both mutant alleles with respect to their wild-type siblings at 24 hpf. F, G, Eye area measured from lateral images of sema6d mutants and their respective wild-type siblings at 24 hpf [unpaired t test, df = 26 (F), df = 21 (G); error bars are SD]. Scale bar: 200 μm.

Expression Data

Gene:	bhlhe40
Fish:	TL sema6ca^ca302/ca302 (TL) sema6ca^ca303/ca303 (TL)
Anatomical Terms:	presumptive retinal pigmented epithelium somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sema6ca^ca302/ca302 (TL) sema6ca^ca303/ca303 (TL)
Observed In:	eye somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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