FIGURE

FIGURE 5

ID

ZDB-FIG-210518-16

Publication

Cheng et al., 2021 - Unbiased Label-Free Quantitative Proteomics of Cells Expressing Amyotrophic Lateral Sclerosis (ALS) Mutations in CCNF Reveals Activation of the Apoptosis Pathway: A Workflow to Screen Pathogenic Gene Mutations

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FIGURE 5

(A) iPSCs from asymptomatic and symptomatic ALS patients harboring the CCNF S621G mutation were subjected to global proteomics analysis. IPA of the quantitative proteomics data predicted activation of the apoptosis pathway. Red and green annotations refer, respectively, to experimentally measured values. Orange and blue annotations and lines refer, respectively, to predicted activation and inhibition as determined by IPA. Dashed lines indicate an indirect causal relationship (log2 values shown; values shown as 5 and −5, respectively, indicate detection of the presence and absence of a protein and are not measured values). (B) Immunoblot analysis of iPSCs from asymptomatic and symptomatic ALS patients harboring the CCNF S621G mutation compared to healthy controls reveals increased expression of pro-apoptotic proteins Bid and Bax. One-way ANOVA with Dunnett’s multiple comparison test (n = 3).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.