FIGURE 5

FIGURE 5

(A) iPSCs from asymptomatic and symptomatic ALS patients harboring the CCNF S621G mutation were subjected to global proteomics analysis. IPA of the quantitative proteomics data predicted activation of the apoptosis pathway. Red and green annotations refer, respectively, to experimentally measured values. Orange and blue annotations and lines refer, respectively, to predicted activation and inhibition as determined by IPA. Dashed lines indicate an indirect causal relationship (log2 values shown; values shown as 5 and −5, respectively, indicate detection of the presence and absence of a protein and are not measured values). (B) Immunoblot analysis of iPSCs from asymptomatic and symptomatic ALS patients harboring the CCNF S621G mutation compared to healthy controls reveals increased expression of pro-apoptotic proteins Bid and Bax. One-way ANOVA with Dunnett’s multiple comparison test (n = 3).