FIGURE

FIGURE 7

ID

ZDB-FIG-210518-19

Publication

Cheng et al., 2021 - Unbiased Label-Free Quantitative Proteomics of Cells Expressing Amyotrophic Lateral Sclerosis (ALS) Mutations in CCNF Reveals Activation of the Apoptosis Pathway: A Workflow to Screen Pathogenic Gene Mutations

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FIGURE 7

Measurements of aberrant neuron branching and apoptosis activation in zebrafish injected with CCNF gene variants S621G, S195R, R574Q, and wild type. (A) Blinded analysis of the number of aberrant branches in zebrafish expressing cyclin F S621G and S195R, respectively, had 2.01-fold (p-value = 0.006, n = 42) and 2.4-fold (p-value = 0.0011, n = 46) more aberrantly branched motor neurons compared to the wild type (one-way ANOVA). Region of interest (ROI) in zebrafish used for imaging. (B) Representative images of zebrafish expressing blue fluorescent protein in motor neurons used to analyse primary motor axon branching. Examples of aberrant branching indicated by red arrows. (C) Cyclin F S621G and S195R had displayed increased cleaved caspase-3 activity by 2.08-fold (n = 39, p-value = 0.0029 and 2.79-fold (n = 28, p-value = 0.0004) (one-way ANOVA adjusted with Kruskal–Wallis test). (D) Representative images of live zebrafish staining with AO. (E) AO positive cells in S621G and S195R were 1.4-fold (p-value = 0.0496, n = 37) and 1.45-fold (p-value = 0.0115, n = 57) higher, respectively, compared to the wild type (one-way ANOVA). (F) Representative images of zebrafish immunostained with cleaved caspase-3 primary antibody. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.