Fig. 3

(A) Schematic of the light-sheet microscope. (B) Sample z-slices taken within a stack of a huc:GCaMP6S transgenic larvae. Stacks were collected at 1 Hz. Imaging experiments captured either the rostral 2/3rds or caudal 2/3rds of the fish. (C) Arrangement of salt pulses during each experiment. Zebrafish experienced escalating concentrations of NaCl pulses in 5-min blocks. (D) Example calcium signal from an activity unit in the olfactory bulb. (E) Example tail curvature trace during imaging experiment. (F) Average stimulus correlation at each voxel of the Z-brain across 15 fish. (G) Histogram of normalized mutual information in units from each of the sensory ganglia in the Z-brain (error bars: mean ± SEM across fish). (H) Stimulus-triggered responses of top NaCl encoding units from the olfactory epithelia, averaged across fish, and their locations within the Z-brain. Responses are separated into trials where the fish swam (blue) or did not swim (purple). Mean ± SEM across fish. (I) Stimulus-triggered responses of top NaCl encoding units in the nasal neuromast averaged across fish as in (H). (J) Confusion matrices depicting stimulus classification accuracy of support vector machines trained from activity in the olfactory epithelia (top) and nasal neuromast (bottom). (K) Confusion matrices depicting behavioral response classification accuracy of support vector machines trained from activity in the olfactory epithelia (top) and nasal neuromast (bottom).