Pan-Camel and isoform-specific anti-Camel morpholino–mediated LOF differentially affects the formation of RF. a Being generated by the SCO (green, GFP, Tg(ET33-mi2a) and the RF (detected by anti-AFRU antibody, red) and spanning the BVS. It may obtain additional contributions from FO and FP. It extends through the central canal to the posterior end of the spinal cord to be disassembled at the filum terminale. b MO1 blocks the formation of RF (b’) and causes the distortion in the distribution of RF+ material in FO (b”) and FP (b”’). c, d In comparison, the effect of isoform-specific morpholinos MS1 and MS2 is less obvious. All confocal images of Tg(ET33-mi2a) transgenic 48-hpf embryos are shown as lateral views with anterior to the left. a Stack of sections. a’ Selected sections to illustrate the primarily apical distribution of the RF material in the SCO. a”’ and a”” are the same as c and d shown for comparing the effect of MO. a’b Blowup of a’. a”b Blowup of a”. d’b Blowup of d’. d”b Blowup of d”. Asterisks indicate lack of RF, and arrows and arrowheads indicate RF. Abbreviations: fo, flexural organ; fp, floor plate; ft, filum terminale; rf, Reissner fiber; sco, subcommissural organ. Scale bar = 200 μm (upper left-hand side column, a–d), 100 μm (four other upper columns marked ’ to ””), and 50 μm (blowups)
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