FIGURE 4

Calculated track measures. (A) Depiction of distance to the wound. It measured for each frame as the shortest distance from the cell’s current position to the entire line of the wound, i.e., the orthogonal projection to the wound. (B) Depiction of V_AP: velocity in anteroposterior axis direction. The visible part of the spine is taken as the y-axis. (C) Depiction of the net displacement, total displacement, meandering index, and mean speed: the net displacement is the distance of the cell between the first and final time frame. Total displacement is the sum of the net displacement between two successive frames. Meandering index corresponds to the net displacement divided by the total displacement. Mean speed is the total displacement divided by traveled time. Formulas show in Table 1 (Eqs 1–4). (D) Depiction of the construction of the mean squared displacement: the displacement between the first time frame and time frame t from all cells is squared and averaged (see Table 1, Eq. 5).