Fig. 4

Fig. 4. DHA-S disturbed normal metabolism. A and B, The levels of glucose and lactate in H9c2 cell cultural medium supernatant after 24 h exposure of DHA-S at 1 g/L and compared with raw cultural medium.(n = 3, data are represented as mean ± SEM, *, P < 0.05; and ***, P < 0.001, analyzed by Student’s unpaired T test.) C, the relative abundance of amino acids in H9c2 cell culture supernatant compared with height intensity of internal standard. (n = 4, data are represented as mean.) D, the content of triacylglycerol (TG), total cholesterol (T-CHO) and low-density lipoprotein cholesterol (LDL-C) were examined in zebrafish larvae after 24 h exposure of DHA-S at 50 mg/L. (n = 3, each group containing 50 embryos. Data are represented as mean ± SEM. ***, P < 0.001, analyzed by Student’s unpaired T test.) E, the dose dependent protective effect of sodium bicarbonate on zebrafish from pericardial edema induced by DHA-S (100 mg/L). (n = 3, each group containing 25 embryos) Data are represented as mean ± SEM. Representative pictures of DHA-S induced pericardial edema. Scale bar, 500 µm.