FIGURE

Fig. 2

ID

ZDB-FIG-210223-27

Publication

Huang et al., 2021 - Sodium dehydroacetate induces cardiovascular toxicity associated with Ca²⁺ imbalance in zebrafish

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Fig. 2

Fig. 2. DHA-S induced Ca²⁺ deficiency in zebrafish. A, The total whole body Ca²⁺ content in zebrafish after 24 h exposure (48–72 hpf) with or without 50 mg/L DHA-S and exogenous addition of Ca²⁺. Data were analyzed by one-way analysis of variance (ANOVA) test (F (3, 8) = 77.56; P < 0.0001). B, The normal percent of zebrafish after 24 h exposure (48–72 hpf) with or without 50 mg/L DHA-S and exogenous addition of Ca²⁺. Data were analyzed by one-way analysis of variance (ANOVA) test (F (4, 10) = 8.299; P = 0.0032). C, The levels of active 1,25(OH)2D3 (calcitriol, CAT) in zebrafish with or without 50 mg/L DHA-S for 24 h (48–72 hpf) and exogenous addition of CAT. Data were analyzed by one-way analysis of variance (ANOVA) test (F (3, 8) = 66.17; P < 0.0001). D, Relative mRNA levels (to Rpl13) of ion-regulated and vitamin D3-related genes. Data were analyzed by two-tailed, unpaired Student's t-test. In A, B, C and D, n = 3, each group containing more than 35 larvae. All the data are expressed as mean ± SEM. *, P < 0.05; and **, P < 0.01; *** , P < 0.001; and ****, P < 0.0001; untreated control versus treated groups.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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