FIGURE

Figure 4

ID

ZDB-FIG-210123-25

Publication

Almeida et al., 2021 - Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration

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Figure 4

ascl1b-2A-Cre, olig2-2A-Cre and neurod1-2A-Cre Cre recombinase activity in the 3 dpf larval midbrain and hindbrain. F3 or F2 adults were mated to the recombination reporter line ubi:Switch to generate double transgenic embryos. Confocal imaging of ascl1b-2A-Cre; ubi:Switch (a–a′′,b–b′′), control sibling ubi:Switch (c,d), olig2-2A-Cre; ubi:Switch (e–e′′,f–f′′) and neurod1-2A-Cre; ubi:Switch (g–g′′,h–h′′) larval midbrain tectum (t), cerebellum (c) and hindbrain (h). Arrows point to the ventricular zone lining the midbrain and hindbrain ventricles (v). Expression of ascl1b-2A-Cre earlier in brain development leads to nearly all descendant neurons expressing mCherry (a,b). No switching occurs in the absence of Cre (c–c′′,d–d′′). olig2-2A-Cre leads to mCherry expression in a subset of cells in the cerebellum (arrowheads), and neural progenitors lining the hindbrain ventricular zone and their descendants (e,f). In neurod1-2A-Cre mCherry expression is absent from the ventricular zone (v arrows) but present throughout neurons in the tectum, cerebellum (arrowheads) and hindbrain (g,h). Scale bars 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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