FIGURE 8

SA exerts anti-metastatic effects partially via Cav-1/Hif-1α signaling. (A) The levels of Hif-1α, Cav-1, LC3, p62, vimentin, E-cad, and N-cad in MDA-MB-231 cells before or after the transfection of HIF-1A plasmid or siHIF-1A. (B) Representative fluorescence photographs showing autophagic activities of MDA-MB-231 cells transfected with or without HIF-1A plasmid or siHIF1-A. Scale bar: 10 μM. All values represent the mean ± SD (n = 3, **p < 0.01). (C) The levels of Hif-1α, Cav-1, LC3, and p62 in SA-treated MDA-MB-231 cells transfected with HIF-1A plasmid with or without siCAV-1 during hypoxia were measured via western blotting analysis. (D) Autophagic flux was determined by a LC3-GFP-mRFP reporter and migration distances were detected by wound healing assays in SA-treated MDA-MB-231 cells after the indicated genetic modifications. Scale bar: 10 μM. Values represent the mean ± SD (n = 3, **p < 0.01 SA + HIF-1A group vs. SA + HIF-1A group + siCAV-1).