Fig. 7

a Characterization of the runx3 locus. In the first three rows, various chromatin marks are indicated¹⁵. The structures of known transcripts across the runx3 locus are shown below. The positions of candidate DMRs called in G2 sperm DNAs are shown underneath the transcript structures, as are the three candidate DMRs discovered in G4⁺ and G4* sperm DNA. The position of the only annotated runx3 CpG island is indicated above the CpG density track. The filtered coverage across the locus in the three WGBS replicates is indicated as well as the extent of methylation in the CpGs that were evaluated in the comparison of G4⁺ and G4* sperm DNAs. The bottom row indicates the positions of repeat across the locus. Note that the hypermethylated DMR in G4* sperm DNA coincides with a peak in H3K4 methylation. b DNA methylation levels in DMRs associated with runx3 and rptor genes in sperm of G4 males. For runx3, the data pertain to the three replicates of the DMR marked with an asterisk in a; for rptor, the relevant DMR is indicated by an asterisk in Supplementary Fig. 5b. c Combined knock-down of runx3 and rptor by anti-sense oligonucleotides impairs larval T cell development. Each data point represents one animal analyzed at 5 dpf by RNA in situ hybridization. In (b, c), each data point represents one animal; t-test, two-tailed; mean ± s.e.m. Source data are provided as Source Data file.