FIGURE

Fig. S51

ID

ZDB-FIG-200623-6

Publication

Oliveira et al., 2020 - Platinum-triggered Bond-cleavage of Pentynoyl amide and N-propargyl handles for Drug-Activation

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Fig. S51

Cisplatin-mediated prodrug decaging in a colon cancer Zebrafish Xenograft model at 7dpi. HCT116 human CRC cells were fluorescently labelled with lipophilic dye CM-DiI and injected into the PVS of 2 dpf Tg(Fli1:eGFP) zebrafish larvae. Zebrafish xenografts were treated in vivo for 6 consecutive days with: DMSO, Cispt, cFU, pFU, cFU+CisPt and pFU+CisPt. At 7dpi (6dpt), zebrafish xenografts were imaged by stereoscope (A-F) and by confocal microscopy (A’-F’ DAPI plus DiI, A’’-F’’ a maximum projection of activated caspase 3). Quantification of proliferation (mitotic figures, G * P=0.0180, ** P=0.0023, *** P=0.0002), apoptosis (activated caspase3, H * P=0.0119, ** P=0.0068) and tumor size (no of tumor cells, I ** P=0.0010, * cFU+CisPt vs pFU+CisPt P=0.0186, * pFU vs pFU+CisPt P=0.0411). Outcomes are expressed by fold induction (normalized values to controls) as AVG ± SEM. The number of xenografts analyzed is indicated in the representative images and each dot represents one zebrafish xenograft. Statistical analysis was performed using an unpaired t-test. Statistical results: ns>0.05, *P≤0.05, **P≤0.01, ***P≤0.001 and ****P≤0.0001. All images are anterior to the left, posterior to right, dorsal up, and ventral down. Scale bar 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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