ZFIN Image: Oliveira et al., 2020, Fig. S51

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Fig. S51

Cisplatin-mediated prodrug decaging in a colon cancer Zebrafish Xenograft model at 7dpi. HCT116 human CRC cells were fluorescently labelled with lipophilic dye CM-DiI and injected into the PVS of 2 dpf Tg(Fli1:eGFP) zebrafish larvae. Zebrafish xenografts were treated in vivo for 6 consecutive days with: DMSO, Cispt, cFU, pFU, cFU+CisPt and pFU+CisPt. At 7dpi (6dpt), zebrafish xenografts were imaged by stereoscope (A-F) and by confocal microscopy (A’-F’ DAPI plus DiI, A’’-F’’ a maximum projection of activated caspase 3). Quantification of proliferation (mitotic figures, G * P=0.0180, ** P=0.0023, *** P=0.0002), apoptosis (activated caspase3, H * P=0.0119, ** P=0.0068) and tumor size (no of tumor cells, I ** P=0.0010, * cFU+CisPt vs pFU+CisPt P=0.0186, * pFU vs pFU+CisPt P=0.0411). Outcomes are expressed by fold induction (normalized values to controls) as AVG ± SEM. The number of xenografts analyzed is indicated in the representative images and each dot represents one zebrafish xenograft. Statistical analysis was performed using an unpaired t-test. Statistical results: ns>0.05, *P≤0.05, **P≤0.01, ***P≤0.001 and ****P≤0.0001. All images are anterior to the left, posterior to right, dorsal up, and ventral down. Scale bar 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Am. Chem. Soc.