ZFIN Figure: Tyrkalska et al., 2019, Fig. 7

ZFIN ID: ZDB-FIG-200608-27

ZFIN ID: ZDB-FIG-200608-27

Tyrkalska et al., 2019 - Inflammasome Regulates Hematopoiesis through Cleavage of the Master Erythroid Transcription Factor GATA1. Immunity 51(1):50-63.e5 Full text @ Immunity

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibodies:	Ab2-gata1a Ab3-spi1
Fish:	mitfa^w2/w2; mpv17^a9/a9 mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a
Condition:	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor
Knockdown Reagent:	MO2-gata1a
Anatomical Term:	whole organism
Stage:	Protruding-mouth

PHENOTYPE:

Fish:	spint1a^{hi2217Tg/hi2217Tg} c264Tg; sh267Tg spint1a^{hi2217Tg/hi2217Tg}; c264Tg; sh267Tg mitfa^w2/w2; mpv17^a9/a9 spint1a^{hi2217Tg/hi2217Tg}; c264Tg; sh267Tg + CRISPR4-caspa mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a
Condition:	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor
Knockdown Reagents:	CRISPR4-caspa MO2-gata1a
Observed In:	cysteine-type endopeptidase activity involved in apoptotic process integument neutrophil neutrophil nucleate erythrocyte whole organism
Stage:	Protruding-mouth

Fig. 7 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab2-gata1a	WB	mitfa^w2/w2; mpv17^a9/a9	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	whole organism
	WB	mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a	control	Protruding-mouth	whole organism
	WB	mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	whole organism
Ab3-spi1	WB	mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a	control	Protruding-mouth	whole organism
	WB	mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	whole organism

Phenotype Details

Fish	Conditions	Stage	Phenotype
c264Tg; sh267Tg	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	neutrophil decreased amount, abnormal
mitfa^w2/w2; mpv17^a9/a9	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	whole organism Ab2-gata1a labeling increased amount, abnormal
mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a	control	Protruding-mouth	nucleate erythrocyte decreased amount, abnormal
		Protruding-mouth	whole organism Ab2-gata1a labeling decreased amount, abnormal
		Protruding-mouth	whole organism Ab3-spi1 labeling increased amount, abnormal
mitfa^w2/w2; mpv17^a9/a9 + MO2-gata1a	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	nucleate erythrocyte decreased amount, ameliorated
		Protruding-mouth	whole organism Ab2-gata1a labeling amount, ameliorated
		Protruding-mouth	whole organism Ab3-spi1 labeling amount, ameliorated
spint1a^{hi2217Tg/hi2217Tg}	control	Protruding-mouth	cysteine-type endopeptidase activity involved in apoptotic process increased occurrence, abnormal
spint1a^{hi2217Tg/hi2217Tg}; c264Tg; sh267Tg	control	Protruding-mouth	integument neutrophil increased amount, abnormal
		Protruding-mouth	neutrophil increased amount, abnormal
spint1a^{hi2217Tg/hi2217Tg}; c264Tg; sh267Tg	chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	integument neutrophil increased amount, abnormal
		Protruding-mouth	neutrophil amount, ameliorated
spint1a^{hi2217Tg/hi2217Tg}; c264Tg; sh267Tg + CRISPR4-caspa	control	Protruding-mouth	neutrophil amount, ameliorated

Full text @ Immunity