Fig. 2

vhl−/− larvae display retinal abnormalities and impaired visual function. vhl−/−larvae possess A) reduced visual capacity in response to a moving striped stimulus (OKR) n = 3, 12 technical replicates per treatment group per experiment. B) reduced movement in response to changes in light intensity (VMR) and C) VMR chromatographs illustrating average max ON/OFF peaks. n = 3, 12 technical replicates per treatment group per experiment. D) vhl−/− larvae display defects in retinal arrangement at 5 dpf. Representative light microscopy images of retinal sections from i) 4 dpf sibling control, ii) 4 dpf vhl−/− iii) 5 dpf sibling control and iv) extensive retinal damage, thought to be fluid accumulation present in 5 dpf vhl−/− larvae (white arrows). Scale bar = 50 μm. E) Morphometric analysis based on 3 independent measurements from 3 larvae per group. Values represent mean ± standard deviation of retinal layer thickness in μm. No statistically significant differences is observed between retinal layers of sibling and vhl−/− at 4 dpf. The retinal layers analysed include Retinal Pigment Epithelium (RPE), Outer Nuclear Layer (ONL), Outer Plexiform Layer (OPL), Inner Nuclear Layer (INL), Inner plexiform Layer (IPL) and Ganglion Cell Layer.