FIGURE

Figure 4

ID

ZDB-FIG-200306-72

Publication

Laselva et al., 2019 - Activity of lumacaftor is not conserved in zebrafish Cftr bearing the major cystic fibrosis-causing mutation

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

WT‐zCftr and F507del‐zCftr channel activity can be potentiated by VX‐770. A, Functional analysis of WT‐zCftr‐GFP in HEK293 cells using FLIPR assay by 0.1% DMSO or 1 μM FSK in the presence or absence of 1 μM VX‐770, at 37°C. After 10 min activation by FSK, CFTR Inhibitor (CFTR_inh‐172, 10 μM) was added to deactivate CFTR. B, Bar graph shows the mean (±SEM) of the slope of FSK activation at 37°C (n = 4). C, Representative traces of F507del‐zCftr‐GFP function following 24 h preincubation at 27°C and acute activation with 1 μM FSK ± 1 μM VX‐770 at 27°C. D, Bar graph shows the mean (±SEM) of the slope of FSK activation at 27°C (n = 4). (**P < .01, ***P < .001). CFTR, cystic fibrosis transmembrane conductance regulator; FLIPR, fluorometric imaging plate reader; FSK, forskolin; GFP, green fluorescent protein; HEK293, human embryonic kidney 293; WT, wild‐type

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB Bioadv