Figure 4

Figure 4

WT‐zCftr and F507del‐zCftr channel activity can be potentiated by VX‐770. A, Functional analysis of WT‐zCftr‐GFP in HEK293 cells using FLIPR assay by 0.1% DMSO or 1 μM FSK in the presence or absence of 1 μM VX‐770, at 37°C. After 10 min activation by FSK, CFTR Inhibitor (CFTR_inh‐172, 10 μM) was added to deactivate CFTR. B, Bar graph shows the mean (±SEM) of the slope of FSK activation at 37°C (n = 4). C, Representative traces of F507del‐zCftr‐GFP function following 24 h preincubation at 27°C and acute activation with 1 μM FSK ± 1 μM VX‐770 at 27°C. D, Bar graph shows the mean (±SEM) of the slope of FSK activation at 27°C (n = 4). (**P < .01, ***P < .001). CFTR, cystic fibrosis transmembrane conductance regulator; FLIPR, fluorometric imaging plate reader; FSK, forskolin; GFP, green fluorescent protein; HEK293, human embryonic kidney 293; WT, wild‐type