FIGURE

Figure 7.

ID

ZDB-FIG-200210-45

Publication

Nagashima et al., 2019 - Midkine-a is required for cell cycle progression of Müller glia glia during neuronal regeneration in the vertebrate retina

Other Figures

(all 10)

All Figure Page

Back to All Figure Page

Figure 7.

The mdka^mi5001 mutant upregulates “core” transcriptional regulators following photoreceptor death. A–C, qPCR for dedifferentiation markers, ascl1a, stat3, and lin28, at 30 and 36 hpl. Both WT and mdka^mi5001 upregulate ascl1a (A; WT: 30 hpl, p < 0.0001, 36 hpl, p < 0.0001; mdka^mi5001: 30 hpl, p = 0.0004, 36 hpl, p = 0.006, p = 0.0066 relative to WT; F-ratio = 37.5606), lin28 (B; WT: 30 hpl, p < 0.0001, 36 hpl, p = 0.0095; mdka^mi5001: 30 hpl, p < 0.0001, 36 hpl, p = 0.0004; F-ratio = 32.9337), and stat3 (C; WT: 30 hpl, p = 0.0048, 36 hpl, p = 0.0016; mdka^mi5001: 30 hpl, p = 0.0004, 36 hpl, p = 0.0016), following lesion. ANOVA with post hoc Tukey, relative to unlesioned. D, E, Western blot for Stat3 in WT and mdka^mi5001 retinas at 1 dpl. WT: p = 0.0002, mdka^mi5001: p = 0.0004, ANOVA with post hoc Tukey; F-ratio = 40.8763. F, Immunocytochemistry for phosphorylated Stat3 (pStat3) in the WT and mdka^mi5001 mutant. In unlesioned retina, immunosignal for pStat3 is not detected in the inner nuclear layer. Following photoreceptor lesion, Müller glia in the inner nuclear layer upregulate pStat3 in WT, whereas mdka^mi5001 mutants have reduced phosphorylation of Stat3. Scale bar, 30 μm. inl, Inner nuclear layer; ipl, inner plexiform layer. *p < 0.01.

Expression Data

Genes:	ascl1a lin28ab stat3
Antibodies:	Ab1-stat3 Ab3-stat3
Fish:	AB mdka^{mi5001/mi5001} (AB)
Condition:	light damage: eye photoreceptor cell
Anatomical Terms:	regenerating tissue retina retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB mdka^{mi5001/mi5001} (AB)
Condition:	light damage: eye photoreceptor cell
Observed In:	regenerating tissue retina retina
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.