FIGURE

Fig 2

ID
ZDB-FIG-200128-2
Publication
Bhattarai et al., 2020 - Neuron-glia interaction through Serotonin-BDNF-NGFR axis enables regenerative neurogenesis in Alzheimer's model of adult zebrafish brain
Other Figures
All Figure Page
Back to All Figure Page
Fig 2

Serotonin regulates NSC plasticity indirectly through HTR1 signaling in periventiruclar neurons.

(A, B) IHC for S100β and PCNA in control (A) and 5-HT-injected (B) brains. (C) Quantification of proliferating glia in conditions of panels A and B. (D, E) IHC for BrdU and HuC/D for newborn neurons at 14 dpi after BrdU treatment at 2 and 3 dpi (D) and BDNF injection (E). (F) Quantification of newborn neurons. (G) IHC for GFP (driven by glial promoter her4.1) and PCNA in control, IL4-injected, Aβ42-injected, and 5-HT-injected brains. (H) Quantification of proliferating glia in conditions of panel G. (I-K) IHC for her4.1-driven GFP and 5-HT. The composite image (I) and single fluorescent channels for her4.1:GFP (J) and 5-HT (K). (L) ISH for htr1a (panel Lʹ: close-up image of framed region in panel L). (M) ISH for htr1d (panel Mʹ: close-up image of framed region in panel M). (N) IHC for pERK and her4.1-driven GFP in control and 5-HT-injected brains. (O) Quantification of pERK-positive periventricular neurons. (P) Working hypothesis on the indirect regulation of 5-HT on NSC plasticity. n = 4 animals for experiments. Scale bars equal 100 μM. Data are represented as mean ± SEM. See also S3 Fig. See S7 Data for supporting information. Aβ42, amyloid-beta42; BDNF, brain-derived neurotrophic factor; BrdU, 5-bromo-2'-deoxyuridine; dpi, days post injection; GFP, green fluorescent protein; HTR1, 5-hydroxytryptamine receptor 1; HuC/D, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3/4; IHC, immunohistochemistry; IL4, interleukin-4; ISH, in situ hybridization; NSC, neural stem cell; pERK,; phosphorylated extracellular signal-regulated kinase; PCNA, proliferating cell nuclear antigen; PVZ, periventricular zone; S100β, S100 calcium-binding protein B; 5-HT, serotonin.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.