Figure 6—figure supplement 1.

(A) Pole cells are tracked from 25 hpf (left) to 30 hpf (right) as they treadmill from high-curvature to low-curvature (dashed blue curves) tissue regions. Mean curvature of the lumenal surface is used as a measure of the local curvature. (B) Quantification of the cell aspect ratios and mean curvature shows that cells retain their shapes independent of the location on the lumenal surface (n = 10, **p<5.0e-5). Error bars are SD. (C) For the measurement of single-cell data on shape deformations in Figure 6I, mosaically labeled cells were tracked in Tg(actb2:GFP-Hsa.UTRN) before and after puncture. Dextran TexasRed was injected into a single blastomere of 16 cell stage embryos for tracking positions of cells before and after puncture. Confocal 3D image datasets were collected before and after puncture and co-registered. The confocal micrographs show XY, YZ, XZ, and a 3D triplanar view centered on the otic vesicle of a single embryo staged at 30 hpf before (top) and after puncture (bottom). Cells positive for TexasRed fluorescence (examples shown in red, blue, and white arrows) were matched before and after puncture. For each cell, the deformation and fluorescence localization were manually measured using the GoFigure2 software.