Figure 4—figure supplement 1.

In addition to experiments at 30 hpf reported in Figure 4, puncturing was conducted at 25 hpf (A, C, E) and 35 hpf (B, D, F). (A–B) Volume regeneration and establishment of contralateral symmetry after unilateral puncturing. The y-axis plots the difference in lumenal volumes normalized to the unpunctured volume (ΔVlVl). (C–D) Lumenal fluid flux Ω in punctured (blue) and unpunctured (red) vesicles during regeneration showing the increase in Ω in punctured embryos and gradual decay to the unpunctured levels. (E–F) Plot showing Ω as a function of Δ⁢VlVl. The data was pooled across multiple embryos for the punctured (blue) and unpunctured (red) ears throughout their regeneration period until 45 hpf. Ω in punctured ears (blue) is positively correlated with Δ⁢VlVl while unpunctured ears (red) continued growing with an approximately constant flux.