FIGURE

Figure 4.

ID
ZDB-FIG-191230-384
Publication
Cayuso et al., 2019 - Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness
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Figure 4.

Increased tension selectively restores boundary expression of <italic>rfng</italic> in <italic>epha4</italic> and <italic>ephrinb3</italic> mutants.

(A–K) Knockdown of mypt1 increases rfng expression at hindbrain boundaries in control embryos (F) and restores rfng expression at specific boundaries in ephrinb3-/- (G), epha4-/- (H), epha4Δ651 (I) and epha4KD (J) mutants compared to uninjected controls (A–E). (K) Percentage of embryos showing rfng expression at the different boundaries for: control (n = 38); MO-mypt1 (45); epha4a-/- (13); epha4a-/-MO-mypt1 (42); efnb3b-/- (14); efnb3b-/-MO-mypt1 (17); epha4aΔ651 (15); epha4aΔ651 MO-mypt1 (22); epha4aKD (13); epha4aKD MO-mypt1 (27). Dorsal views, anterior to the top. Arrowheads indicate normal boundary expression of rfng, while asterisks indicate reduction or absence of rfng expression at boundaries. Scale bar: 50 μm.
Expression Data
Gene:
Fish: (all 10)
Knockdown Reagent:
Anatomical Terms: (all 6)
Stage: 20-25 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish: (all 7)
Knockdown Reagent:
Observed In: (all 6)
Stage: 20-25 somites

Phenotype Detail
Acknowledgments
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