FIGURE

Fig. 3.

ID

ZDB-FIG-191230-1891

Publication

Liu et al., 2019 - Combinatorial genetic replenishments in myocardial and outflow tract tissues restore heart function in tnnt2 mutant zebrafish

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Fig. 3.

Gene expression in OFT is deregulated in tnnt2a mutant zebrafish with myocardial tnnt2a supplementation. (A) Workflow of sample collections for whole-fish RNA-seq of four groups: tnnt2^+/−, tnnt2a^−/− Tg(myl7:tnnt2a); tnnt2a^−/− and Tg(myl7:tnnt2a); tnnt2a^+/−. (B) Volcano plot of differentially expressed genes in tnnt2a^+/− versus Tg(myl7:tnnt2a); tnnt2a^−/−. (C,D) Sarcomere- and OFT-relevant gene expression in dissected heart of tnnt2a^+/+, tnnt2a^−/−, and Tg(myl7:tnnt2a); tnnt2a^−/− after DOX induction at 16 hpf. *P<0.05, ***P<0.001, ****P<0.0001, NS, not significant; n=30 per group. Statistical differences were evaluated using two-way ANOVA. Red asterisks, sarcomere genes in Tg(myl7:tnnt2a); tnnt2a^−/− can almost return to wild-type level after DOX induction; red hashes, OFT-relevant genes in Tg(myl7:tnnt2a); tnnt2a^−/− are more deviated from wild-type level after DOX induction compared with tnnt2a^−/−.

Expression Data

Genes:	actc1a bmp2b gata4 gata5 has2 mKate2 myl7 tbx2b tbx20 tnnc1a tnni1b tnnt2a tpm4a
Fish:	fdu106Tg tnnt2a^{fdu108/fdu108}; fdu106Tg
Condition:	chemical treatment by environment: doxycycline
Anatomical Term:	heart
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tnnt2a^{fdu108/fdu108}; fdu106Tg
Condition:	chemical treatment by environment: doxycycline
Observed In:	heart
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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