FIGURE

Figure 7

ID

ZDB-FIG-191230-1591

Publication

Morishita et al., 2019 - A critical role of VMP1 in lipoprotein secretion

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Figure 7

Neutral lipids accumulate within the ER membrane in the absence of VMP1.

(A–C) Transmission electron microscopy of intestinal epithelial cells (A) and hepatocytes (B) from 6-dpf vmp1^+/- and vmp1^-/- zebrafish and VMP1-depleted HepG2 cells (C). Black and white arrowheads indicate the presence and absence of a lipid bilayer on neutral lipid-containing structures, respectively. Arrows indicate the ER membrane. Data are representative of three independent experiments. Scale bars, 500 nm and 100 nm in magnified panels. (D) Models for the membrane structure on lipids in the ER in wild-type and VMP1-deficient cells. Black and white arrowheads correspond to those in (A) to (C). In VMP1-deficient cells, the surfaces of neutral lipid structures (monolayer) are continuous to the ER membranes (bilayer), whereas only phospholipid monolayers cover neutral lipid structures in normal cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vmp1^jt3/jt3 (RW)
Observed In:	hepatocyte endoplasmic reticulum membrane hepatocyte lipoprotein transport intestinal epithelial cell endoplasmic reticulum membrane intestinal epithelial cell lipoprotein transport
Stage:	Day 6

Phenotype Detail

Acknowledgments

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