FIGURE

Fig 8

ID

ZDB-FIG-191230-1468

Publication

Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b

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Fig 8

Marcksb interacts with Hsp70.3 to regulate the dorsoventral patterning and the extracellular level of Bmp2b.

(A) Morphological phenotypes of embryos at 12 hpf, injected with sub-dosages of morpholinos against marcksb or hsp70 or the combination of both (marcksb_MO: 3 ng/embryo; hsp70_MO: 2 ng/embryo). (B-E) WISH analysis of szl (B), ved (C), otx2 (D), foxi1 (E). The percentage of embryos with different phenotypes for each group were indicated in the graph. Dosages of morpholino per embryo are indicated in the figure panel. For szl, ved and otx2, embryos are animal-pole view with dorsal to the right; for foxi1, embryos are lateral view with dorsal to the right. “n” represents the number of embryos we observed. (F) Quantitative measurement of secreted Bmp2b. The data were presented as scatter plots with median; “*”: P < 0.01, from Student’s t-test.

Expression Data

Genes:	foxi1 otx2b szl ved
Fish:	AB AB + MO1-marcksb AB + MO1-hsp70.3 AB + MO1-hsp70.3 + MO1-marcksb
Knockdown Reagents:	MO1-hsp70.3 MO1-marcksb
Anatomical Terms:	neuroectoderm non neural ectoderm whole organism ventral region
Stage Range:	Shield to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-marcksb AB + MO1-hsp70.3 AB + MO1-hsp70.3 + MO1-marcksb
Knockdown Reagents:	MO1-hsp70.3 MO1-marcksb
Observed In:	neuroectoderm non neural ectoderm whole organism whole organism ventral region
Stage Range:	Shield to 5-9 somites

Phenotype Detail

Acknowledgments

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