FIGURE

Fig. 6

ID

ZDB-FIG-190826-11

Publication

Kague et al., 2019 - Scleraxis genes are required for normal musculoskeletal development and for rib growth and mineralization in zebrafish

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Fig. 6

Scxa mutants have reduced levels of tendon markers, but somitic MTJs appear normal. A, B) In situ hybridization for xirp2a at 52 hpf (A) and tnmd at 3 dpf (B) for scxa^−/− and their siblings (+/+), lateral view, anterior to left. B) The main image shows the anterior somites, inset-whole embryo. Both xirp2a and tnmd mRNA levels are reduced at the MTJs at the somitic borders (arrowheads). C) Confocal stacks of immunodetection of MyHC (A4.1025) and Tsp4b in somites 11–14 of 4 dpf embryos of scxa^−/− and siblings showing normal distribution of Tsp4b and normal muscle structure. All scale bars, 100 µm.

Expression Data

Genes:	tnmd xirp2a
Antibodies:	Ab-A4.1025 Ab4-thbs4b
Fish:	AB scxa^kg170/kg170 (AB) scxb^kg107/kg107 (AB)
Anatomical Terms:	myotome skeletal muscle cell vertical myoseptum muscle tendon junction
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	scxa^kg170/kg170 (AB) scxb^kg107/kg107 (AB)
Observed In:	myotome vertical myoseptum muscle tendon junction
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.