Fig. 1
- ID
- ZDB-FIG-190812-1
- Publication
- Zhang et al., 2019 - Müller Glial Cells Participate in Retinal Waves via Glutamate Transporters and AMPA Receptors
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Müller Glial Cells of Zebrafish Larvae Exhibit Spontaneous Rhythmic Electrical Activities (A) Left, schematic of vertebrate retinal cellular structure. Right, confocal images showing the morphology of a Müller glial cell (MGC) when 1% rhodamine (red) was loaded into the MGC via a whole-cell recording pipette in a 3-dpf Tg(GFAP:eGFP) larva. AC, amacrine cell; BC, bipolar cell; HC, horizontal cell; IPL, inner plexiform layer; MGC, Müller glial cell; ONL, outer nuclear layer; OPL, outer plexiform layer; PhR, photoreceptor; RGC, retinal ganglion cell. (B) Spontaneous rhythmic giant activities of an MGC monitored with whole-cell recording in a 3-dpf larva under current- (top) or voltage-clamp mode (at −60 mV, bottom). (C) Correlated spontaneous giant activities between an MGC and an RGC monitored with double whole-cell recordings. Left, enlarged view of the correlated events shown in the dashed boxes. (D) Cross-correlation between MGCs’ and RGCs’ spontaneous giant activities. The 34 events obtained from 11 pairs of 1 MGC and 1 RGC were analyzed. The gray lines represent the cross-correlation of single events, and the red line indicates the average. (E) Correlated spontaneous electrical activities between two nearby MGCs monitored with double whole-cell recordings. Both cells were held at −60 mV. (F) Cross-correlation between MGCs’ spontaneous giant activities. The 16 events obtained from 5 MGC pairs were analyzed. The gray lines represent the cross-correlation of single events, and the red line indicates the average. (G) Temporal distribution of the occurrence of MGCs’ spontaneous giant electrical activities. Whole-cell recording data were obtained from 49 larvae. |