PUBLICATION
Müller Glial Cells Participate in Retinal Waves via Glutamate Transporters and AMPA Receptors
- Authors
- Zhang, R.W., Du, W.J., Prober, D.A., Du, J.L.
- ID
- ZDB-PUB-190606-8
- Date
- 2019
- Source
- Cell Reports 27: 2871-2880.e2 (Journal)
- Registered Authors
- Du, Jiu Lin, Prober, David
- Keywords
- AMPA receptor, Müller glial cell, glutamate transporter, retinal wave, zebrafish
- MeSH Terms
-
- Amacrine Cells/metabolism
- Amacrine Cells/physiology
- Amino Acid Transport System X-AG/antagonists & inhibitors
- Amino Acid Transport System X-AG/genetics
- Amino Acid Transport System X-AG/metabolism*
- Animals
- Animals, Genetically Modified
- Calcium/metabolism*
- Ependymoglial Cells/cytology
- Ependymoglial Cells/drug effects
- Ependymoglial Cells/metabolism*
- Ependymoglial Cells/physiology
- Glutamic Acid/metabolism*
- Glutamic Acid/pharmacology
- Larva/drug effects
- Larva/metabolism
- Larva/physiology
- Neuroglia/cytology
- Neuroglia/metabolism*
- Neuroglia/physiology
- Receptors, AMPA/antagonists & inhibitors
- Receptors, AMPA/metabolism*
- Retina/cytology
- Retina/metabolism
- Retina/physiology
- Retinal Bipolar Cells/metabolism
- Retinal Bipolar Cells/physiology
- Retinal Ganglion Cells/drug effects
- Retinal Ganglion Cells/metabolism*
- Retinal Ganglion Cells/physiology
- Zebrafish
- PubMed
- 31167134 Full text @ Cell Rep.
Citation
Zhang, R.W., Du, W.J., Prober, D.A., Du, J.L. (2019) Müller Glial Cells Participate in Retinal Waves via Glutamate Transporters and AMPA Receptors. Cell Reports. 27:2871-2880.e2.
Abstract
Retinal waves, the spontaneous patterned neural activities propagating among developing retinal ganglion cells (RGCs), instruct the activity-dependent refinement of visuotopic maps. Although it is known that the wave is initiated successively by amacrine cells and bipolar cells, the behavior and function of glia in retinal waves remain unclear. Using multiple in vivo methods in larval zebrafish, we found that Müller glial cells (MGCs) display wave-like spontaneous activities, which start at MGC processes within the inner plexiform layer, vertically spread to their somata and endfeet, and horizontally propagate into neighboring MGCs. MGC waves depend on glutamatergic signaling derived from bipolar cells. Moreover, MGCs express both glia-specific glutamate transporters and the AMPA subtype of glutamate receptors. The AMPA receptors mediate MGC calcium activities during retinal waves, whereas the glutamate transporters modulate the occurrence of retinal waves. Thus, MGCs can sense and regulate retinal waves via AMPA receptors and glutamate transporters, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping