PUBLICATION

Müller Glial Cells Participate in Retinal Waves via Glutamate Transporters and AMPA Receptors

Authors
Zhang, R.W., Du, W.J., Prober, D.A., Du, J.L.
ID
ZDB-PUB-190606-8
Date
2019
Source
Cell Reports   27: 2871-2880.e2 (Journal)
Registered Authors
Du, Jiu Lin, Prober, David
Keywords
AMPA receptor, Müller glial cell, glutamate transporter, retinal wave, zebrafish
MeSH Terms
  • Amacrine Cells/metabolism
  • Amacrine Cells/physiology
  • Amino Acid Transport System X-AG/antagonists & inhibitors
  • Amino Acid Transport System X-AG/genetics
  • Amino Acid Transport System X-AG/metabolism*
  • Animals
  • Animals, Genetically Modified
  • Calcium/metabolism*
  • Ependymoglial Cells/cytology
  • Ependymoglial Cells/drug effects
  • Ependymoglial Cells/metabolism*
  • Ependymoglial Cells/physiology
  • Glutamic Acid/metabolism*
  • Glutamic Acid/pharmacology
  • Larva/drug effects
  • Larva/metabolism
  • Larva/physiology
  • Neuroglia/cytology
  • Neuroglia/metabolism*
  • Neuroglia/physiology
  • Receptors, AMPA/antagonists & inhibitors
  • Receptors, AMPA/metabolism*
  • Retina/cytology
  • Retina/metabolism
  • Retina/physiology
  • Retinal Bipolar Cells/metabolism
  • Retinal Bipolar Cells/physiology
  • Retinal Ganglion Cells/drug effects
  • Retinal Ganglion Cells/metabolism*
  • Retinal Ganglion Cells/physiology
  • Zebrafish
PubMed
31167134 Full text @ Cell Rep.
Abstract
Retinal waves, the spontaneous patterned neural activities propagating among developing retinal ganglion cells (RGCs), instruct the activity-dependent refinement of visuotopic maps. Although it is known that the wave is initiated successively by amacrine cells and bipolar cells, the behavior and function of glia in retinal waves remain unclear. Using multiple in vivo methods in larval zebrafish, we found that Müller glial cells (MGCs) display wave-like spontaneous activities, which start at MGC processes within the inner plexiform layer, vertically spread to their somata and endfeet, and horizontally propagate into neighboring MGCs. MGC waves depend on glutamatergic signaling derived from bipolar cells. Moreover, MGCs express both glia-specific glutamate transporters and the AMPA subtype of glutamate receptors. The AMPA receptors mediate MGC calcium activities during retinal waves, whereas the glutamate transporters modulate the occurrence of retinal waves. Thus, MGCs can sense and regulate retinal waves via AMPA receptors and glutamate transporters, respectively.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes