Fig. S8
- ID
- ZDB-FIG-190729-5
- Publication
- Jones et al., 2019 - Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro
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Bifunctional compounds do not enhance phagocytosis of conidia by wild-type zebrafish neutrophils. A) Diagram of a 72 hpf zebrafish larva indicating the site of inoculation and the site of analysis (i). (ii) Collapsed z-stack (maximum intensity) representative image showing conidia (blue, Hoechst) and neutrophils (green, GFP) in the caudal venous plexus of Tg(mpx:GFP/mpeg1:mCherry) embryos injected with spi1-MO at the one-cell stage and infected with A. fumigatus conidia at 72 hpi. Scale: 100 μm. iii) highermagnification of neutrophils and conidia indicating extracellular conidia (open yellow arrowhead) and examples of phagocytosis by neutrophils (filled yellow arrowheads). Scale: 20 μm. B) Graph shows neutrophil (N) and macrophage (M) counts in each caudal venous plexus field of view (FOV) for spi1-MO morphant larvae injected with treated and control conidia. C) Graph shows conidia counts per FOV for each treatment group. D) Graph shows the percent of neutrophils containing conidia at 2 hpi for each treatment group. E) Graph shows the number of phagocytosed conidia per FOV at 2 hpi for each treatment group. Each point represents an infected larva. |