FIGURE

Fig. S8

ID

ZDB-FIG-190729-5

Publication

Jones et al., 2019 - Bifunctional Small Molecules Enhance Neutrophil Activities Against Aspergillus fumigatus in vivo and in vitro

Other Figures

All Figure Page

Back to All Figure Page

Fig. S8

Bifunctional compounds do not enhance phagocytosis of conidia by

wild-type zebrafish neutrophils.

A) Diagram of a 72 hpf zebrafish larva indicating the site of inoculation and the site of

analysis (i). (ii) Collapsed z-stack (maximum intensity) representative image showing

conidia (blue, Hoechst) and neutrophils (green, GFP) in the caudal venous plexus of

Tg(mpx:GFP/mpeg1:mCherry) embryos injected with spi1-MO at the one-cell stage

and infected with A. fumigatus conidia at 72 hpi. Scale: 100 μm. iii) highermagnification

of neutrophils and conidia indicating extracellular conidia (open yellow

arrowhead) and examples of phagocytosis by neutrophils (filled yellow arrowheads).

Scale: 20 μm.

B) Graph shows neutrophil (N) and macrophage (M) counts in each caudal venous plexus

field of view (FOV) for spi1-MO morphant larvae injected with treated and control

conidia.

C) Graph shows conidia counts per FOV for each treatment group.

D) Graph shows the percent of neutrophils containing conidia at 2 hpi for each treatment

group.

E) Graph shows the number of phagocytosed conidia per FOV at 2 hpi for each treatment

group. Each point represents an infected larva.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol