FIGURE

Figure 3

ID

ZDB-FIG-190723-918

Publication

Syed et al., 2019 - Expanding the Zebrafish Genetic Code through Site-Specific Introduction of Azido-lysine, Bicyclononyne-lysine, and Diazirine-lysine

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Figure 3

The site-specific incorporation of bicylononyne-lysine and diazirine-lysine in the zebrafish embryos. (A) The Brightfield (left) or fluorescent (right) images of the zebrafish embryos injected with mRNA(s) for LifeAct-eGFP Y39* with the orthogonal tRNA^Pyl-CUA -mRNA aaRS for AzK/BCNK with (top panel) BCNK. Shown also are fields with multiple examples of each of the zebrafish embryos injected with mRNA(s) for LifeAct-eGFP Y39* with the orthogonal tRNA^Pyl-CUA -mRNA aaRS for AbK, with (middle panel) or without (bottom panel) AbK. (B) The localization of LifeAct-eGFP Y39BCNK (top panel) and LifeAct-eGFP Y39AbK to cell-cell junctions and cortical speckles in the dechorionized embryos injected as described in (A). (C) Shown in the top panel is an IP-Western blot from the zebrafish embryos injected with mRNA(s) for GST (left lane) or GFT F52* with the orthogonal tRNA^Pyl-CUA -aaRS pair for AzK/BCNK, without (middle lane) or with (right lane) BCNK. The bottom panel shows a similar experiment showing the incorporation of AbK into the zebrafish embryos using the appropriate amino-acid and the tRNA^Pyl-CUA –aaRS cocktail for AbK. The immunoprecipitations and Western blots were performed with antibodies against GST.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.