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Figure 3

ID
ZDB-IMAGE-190723-963
Source
Figures for Syed et al., 2019
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Figure Caption

Figure 3

The site-specific incorporation of bicylononyne-lysine and diazirine-lysine in the zebrafish embryos. (A) The Brightfield (left) or fluorescent (right) images of the zebrafish embryos injected with mRNA(s) for LifeAct-eGFP Y39* with the orthogonal tRNAPyl-CUA -mRNA aaRS for AzK/BCNK with (top panel) BCNK. Shown also are fields with multiple examples of each of the zebrafish embryos injected with mRNA(s) for LifeAct-eGFP Y39* with the orthogonal tRNAPyl-CUA -mRNA aaRS for AbK, with (middle panel) or without (bottom panel) AbK. (B) The localization of LifeAct-eGFP Y39BCNK (top panel) and LifeAct-eGFP Y39AbK to cell-cell junctions and cortical speckles in the dechorionized embryos injected as described in (A). (C) Shown in the top panel is an IP-Western blot from the zebrafish embryos injected with mRNA(s) for GST (left lane) or GFT F52* with the orthogonal tRNAPyl-CUA -aaRS pair for AzK/BCNK, without (middle lane) or with (right lane) BCNK. The bottom panel shows a similar experiment showing the incorporation of AbK into the zebrafish embryos using the appropriate amino-acid and the tRNAPyl-CUA –aaRS cocktail for AbK. The immunoprecipitations and Western blots were performed with antibodies against GST.

Acknowledgments
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