Fig. S4

Loss of zfpm2a and zfpm2b in zebrafish did not cause defects in cardiac chamber development. (A) The zebrafish zfpm2a genomic locus. Exons (blue boxes) are numbered. The transposon is inserted after the first intron. Inset panel, fusion GFP expression in embryonic heart at 72 hpf. Lower panel, schematic representations of the domain structure of the zebrafish Zfpm2a protein. Insertion of the gene-trapping element results in a fusion transcript that will be translated into a fusion protein containing the first 13 amino acids from Zfpm2a at the 7 N-terminus. (B) The zebrafish zfpm1 genomic locus. Deletions in Δ23 allele introduced by CRISPY/Cas9 targeting are shown as dashes. Lower panel, schematic representations of the domain structure of the zebrafish Zfpm2b protein and truncated protein derived from the Δ23 allele. (C) Live images of control and zfpm2a-/-; zfpm2b-/- zebrafish embryos at designated time points. Lateral view, anterior to the left. (C) Left panels, live images of control and zfpm2a-/-, zfpm2b-/- zebrafish at 60 dpf. Lateral view, anterior to the left. Middle panels, representative images of dissected hearts from zfpm2a-/-; zfpm2b-/- and WT sibling fish at 60 dpf. Right panels, histological analysis of hearts of zfpm2a-/-; zfpm2b-/- and WT sibling fish. Sections were Masson's trichrome stained. Scale bars: 200μm.