ZFIN ID: ZDB-FIG-190611-28
Turner et al., 2019 - Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits. PLoS One   14:e0211073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 2

slbpty77e mutants have less neurons and show axonal defects.

(A-D”) Acetylated α-tubulin labelling of wildtype (A-D) and ele mutant (A’-D”) embryo brains and eyes. Frontal (A-A’; D-D”) and lateral (B- B’) views of brains and lateral views of eyes (C,C’) of 3dpf (A-C’) and 30hpf (D- D”) wildtype (A-D), slbpty77e mutant (A’-D’) and morphant (D”) embryos. Arrowhead in C’ highlights the aberrant extension of RGC axons within the retina. The asterisks in D’-D” highlight aberrantly positioned axons near the midline commissural region in slbp mutant and morphant embryos. (E-E’) Lateral view with anterior to the left of 3dpf wildtype (E) and slbpty77e mutant (E’) embryos showing expression of the Tg(-8.4neurog1:GFP)sb1 transgene (green) labelled neurons and acetylated α-acetylated tubulin labelled axons/neurites (magenta). (F-F’) Frontal views of the telencephalon in 3dpf wildtype (F) and slbpty77e mutant (F’) embryos showing expression of the Tg(lhx5:GFP)b1205 transgene (green) labelled neurons, SV2 labelled neuropil (red) and acetylated α-acetylated tubulin labelled axons/neurites (cyan). Abbreviations: AC, anterior commissure; POC, post-optic commissure; ON, optic nerve; T, telencephalon; PC, posterior commissure; OT, optic tectum; Hb,habenula; E,eye; P,pineal. Scale bars: (A-C”, E, E’) 100μm (D-D”, F, F’) 50μm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP Ab3-GFP b1205Tg standard conditions Protruding-mouth telencephalon neuron IHC
Ab3-GFP sb1Tg standard conditions Protruding-mouth epiphysis IHC
Ab3-GFP Protruding-mouth habenula IHC
Ab3-GFP Protruding-mouth telencephalon IHC
Ab3-GFP slbpty77e/ty77e; b1205Tg standard conditions Protruding-mouth telencephalon neuron IHC
Ab3-GFP slbpty77e/ty77e; sb1Tg standard conditions Protruding-mouth epiphysis IHC
Ab3-GFP Protruding-mouth telencephalon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-SV2 IHC b1205Tg standard conditions Protruding-mouth tectal neuropile
IHC slbpty77e/ty77e; b1205Tg standard conditions Protruding-mouth telencephalon
Ab1-tuba IHC WT standard conditions Prim-15 anterior commissure
IHC Prim-15 postoptic commissure
IHC Protruding-mouth anterior commissure
IHC Protruding-mouth caudal commissure
IHC Protruding-mouth cranial nerve II
IHC Protruding-mouth optic tectum
IHC Protruding-mouth postoptic commissure
IHC Protruding-mouth retinal ganglion cell axon
IHC Protruding-mouth telencephalon
IHC WT + MO1-slbp standard conditions Prim-15 anterior commissure
IHC Prim-15 postoptic commissure
IHC b1205Tg standard conditions Protruding-mouth telencephalon axon
IHC sb1Tg standard conditions Protruding-mouth habenula axon
IHC Protruding-mouth optic tectum axon
IHC Protruding-mouth telencephalon axon
IHC slbpty77e/ty77e standard conditions Prim-15 anterior commissure
IHC Prim-15 postoptic commissure
IHC Protruding-mouth anterior commissure
IHC Protruding-mouth caudal commissure
IHC Protruding-mouth Not Detected cranial nerve II
IHC Protruding-mouth Not Detected optic tectum
IHC Protruding-mouth postoptic commissure
IHC Protruding-mouth telencephalon
IHC slbpty77e/ty77e; b1205Tg standard conditions Protruding-mouth telencephalon axon
IHC slbpty77e/ty77e; sb1Tg standard conditions Protruding-mouth habenula axon
IHC Protruding-mouth Not Detected optic tectum
IHC Protruding-mouth telencephalon axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slbp standard conditions Prim-15 anterior commissure ab1-tuba labeling decreased amount, abnormal
Prim-15 anterior commissure absent, abnormal
Prim-15 anterior commissure axon mislocalised, abnormal
Prim-15 anterior commissure decreased size, abnormal
Prim-15 anterior commissure morphology, abnormal
Prim-15 postoptic commissure ab1-tuba labeling decreased amount, abnormal
Prim-15 postoptic commissure decreased size, abnormal
Prim-15 postoptic commissure morphology, abnormal
slbpty77e/ty77e standard conditions Prim-15 anterior commissure ab1-tuba labeling decreased amount, abnormal
Prim-15 anterior commissure absent, abnormal
Prim-15 anterior commissure axon mislocalised, abnormal
Prim-15 anterior commissure decreased size, abnormal
Prim-15 anterior commissure morphology, abnormal
Prim-15 postoptic commissure ab1-tuba labeling decreased amount, abnormal
Prim-15 postoptic commissure decreased size, abnormal
Prim-15 postoptic commissure defasciculated, abnormal
Prim-15 postoptic commissure morphology, abnormal
Protruding-mouth anterior commissure ab1-tuba labeling decreased amount, abnormal
Protruding-mouth anterior commissure axon mislocalised, abnormal
Protruding-mouth anterior commissure decreased size, abnormal
Protruding-mouth anterior commissure morphology, abnormal
Protruding-mouth caudal commissure ab1-tuba labeling decreased amount, abnormal
Protruding-mouth cranial nerve II ab1-tuba labeling absent, abnormal
Protruding-mouth cranial nerve II decreased size, abnormal
Protruding-mouth habenular commissure absent, abnormal
Protruding-mouth optic tectum ab1-tuba labeling absent, abnormal
Protruding-mouth postoptic commissure ab1-tuba labeling decreased amount, abnormal
Protruding-mouth postoptic commissure axon mislocalised, abnormal
Protruding-mouth postoptic commissure decreased size, abnormal
Protruding-mouth postoptic commissure morphology, abnormal
Protruding-mouth retinal ganglion cell axon guidance process quality, abnormal
Protruding-mouth retinal ganglion cell axon mislocalised, abnormal
Protruding-mouth retinal ganglion cell axon misrouted, abnormal
Protruding-mouth tectal neuropile decreased size, abnormal
Protruding-mouth telencephalon ab1-tuba labeling decreased amount, abnormal
slbpty77e/ty77e; b1205Tg standard conditions Protruding-mouth telencephalon ab-sv2 labeling decreased amount, abnormal
Protruding-mouth telencephalon axon ab1-tuba labeling decreased amount, abnormal
Protruding-mouth telencephalon neuron GFP expression decreased amount, abnormal
slbpty77e/ty77e; sb1Tg standard conditions Protruding-mouth habenula axon ab1-tuba labeling decreased amount, abnormal
Protruding-mouth optic tectum ab1-tuba labeling absent, abnormal
Protruding-mouth telencephalon GFP expression decreased amount, abnormal
Acknowledgments:
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