FIGURE

Fig. 4

ID

ZDB-FIG-190108-17

Publication

Lin et al., 2018 - Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

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Fig. 4

3-dimensional renderings of embedded specimens. (A) Reconstructed 3 dpf zebrafish larva at 0.743 × 0.743 × 0.743 µm³ voxel resolution. (B) Reconstructed adult Daphnia (3 × 3 × 3 µm³ voxel resolution). (C) Reconstructed adult Drosophila (3.1 × 3.1 × 3.1 µm³ voxel resolution). Digital cross-sections can be generated at any angle as shown on the left column. Surface renderings are shown on the right, rendered using commercial software. Embedding resin can be seen in all scans outside of the sample (noted by *), but does not interfere with the sample itself. The zebrafish larva was imaged at Argonne National Laboratory at the Advanced Photon Source that is synchrotron-based. The Daphnia or Drosophila specimens were imaged with a commercial X-ray microscope.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Vis. Exp.