FIGURE

Fig. 3

ID

ZDB-FIG-190108-16

Publication

Lin et al., 2018 - Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Other Figures

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Fig. 3

A wide variety of specimens can be embedded with our protocol. (A) 7 dpf zebrafish larva. (B) Adult Drosophila. (C) Adult Daphnia. (D) Mouse embryo. Larger samples such as the mouse embryo are accommodated by a few modifications to the protocol. Briefly, the polyimide tubing was filled to 1/3 of its length with resin and polymerized prior to embedding. Fixed and stained sample was placed on top of the pre-polymerized resin. The tubing was then filled with un-polymerized resin followed by a second polymerization. The polyimide tubing was removed prior to photography to allow better visualization of the sample in this figure. The removal of the tubing is not necessary for successful image acquisition by micro-CT. Scale bars: specimen images, 5 mm; enlarged insets, 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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