ZFIN ID: ZDB-FIG-181108-14
Ranski et al., 2018 - Characterization of retinal regeneration in adult zebrafish following multiple rounds of phototoxic lesion. PeerJ   6:e5646 Full text @ Peer J.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 4

Following six rounds of light treatment Müller glia remain persistently gliotic at the canonical peak stage of progenitor proliferation and migration.(A–I) Retinal sections collected at 72 hpL immunolabeled with anti-GFAP (red) and anti-PCNA (green) in untreated control (No Light Treatment), experimental control (1×Light Treatment), and experimental retinas (1×Light Treatment). (A–C) Müller glia are immunolabeled with anti-GFAP. (D–F) Müller glia that have re-entered the cell cycle are immunolabeled with anti-PCNA. (G–I) Merge of anti-GFAP and anti-PCNA immunolabeling. (J) Graph showing the average fold-change in expression of genes associated with (1) general proliferation (cdk1, c-jun), (2) retinal regeneration (shha, shhb, pcna, pax6a, pax6b, six3b and tgif1), (3) Müller cell function (glulb, gfap, kcnj10a, rlbp1b, vim), and (4) RPE function (rlbp1a). Asterisk indicates significantly different from control (p < 0.05). Scale bar represents 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdk1 WT light ablation: photoreceptor cell Adult Muller cell RTPCR
gfap WT light ablation: photoreceptor cell Adult Muller cell RTPCR
glulb WT light ablation: photoreceptor cell Adult Muller cell RTPCR
jun WT light ablation: photoreceptor cell Adult Muller cell RTPCR
kcnj10a WT light ablation: photoreceptor cell Adult Muller cell RTPCR
pax6a WT light ablation: photoreceptor cell Adult Muller cell RTPCR
pax6b WT light ablation: photoreceptor cell Adult Muller cell RTPCR
pcna WT light ablation: photoreceptor cell Adult Muller cell RTPCR
rlbp1a WT light ablation: photoreceptor cell Adult Muller cell RTPCR
rlbp1b WT light ablation: photoreceptor cell Adult Muller cell RTPCR
shha WT light ablation: photoreceptor cell Adult Muller cell RTPCR
shhb WT light ablation: photoreceptor cell Adult Muller cell RTPCR
six3b WT light ablation: photoreceptor cell Adult Muller cell RTPCR
tgif1 WT light ablation: photoreceptor cell Adult Muller cell RTPCR
vim WT light ablation: photoreceptor cell Adult Muller cell RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT light ablation: photoreceptor cell Adult Muller cell cdk1 expression decreased amount, abnormal
Adult Muller cell glulb expression decreased amount, abnormal
Adult Muller cell pax6b expression increased amount, abnormal
Adult Muller cell pcna expression decreased amount, abnormal
Adult Muller cell rlbp1b expression decreased amount, abnormal
Adult Muller cell shha expression increased amount, abnormal
Adult Muller cell shhb expression decreased amount, abnormal
Adult Muller cell six3b expression decreased amount, abnormal
Adult Muller cell tgif1 expression decreased amount, abnormal
Adult Muller cell vim expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Peer J.