FIGURE

Fig. S7

ID

ZDB-FIG-180828-66

Publication

Tsai et al., 2018 - Small molecule inhibition of RAS/MAPK signaling ameliorates developmental pathologies of Kabuki Syndrome

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Fig. S7

Treatment with dmDf rescues the developmental defects of kmt2d-CRISPR F0 mutants. (a) Similar to kmt2d-MO, kmt2d-CRISPR F0s also exhibit CE defects, and treatment with dmDf ameliorates the CE defects in kmt2d-CRISPR F0 embryos. (b) 5 dpf embryos were stained with Alcian blue to visualize jaw layout. kmt2d-CRISPR F0s exhibit the same change in jaw layout as kmt2d-MO-injected embryos, with a reduced distance between MK and CH cartilages. Treatment with dmDf ameliorates the jaw defects seen in kmt2d-CRISPR F0 embryos. (c) Quantitative measurement of the distance between MK and CH cartilages. ***: p< 0.001. Error bars show SEM (standard error of the mean).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + CRISPR10-kmt2d
Condition:	chemical treatment by environment: B-Raf inhibitor
Knockdown Reagent:	CRISPR10-kmt2d
Observed In:	ceratohyal cartilage convergent extension involved in gastrulation mandibular arch skeleton Meckel's cartilage
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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