Fig. S1

Proportion and patterning of zebrafish fins is enhanced by calcineurin inhibition.

Fin growth occurs through sequential addition of a regular pattern of lepidotrichia hemi-ray dermal bone segments at growing end of the fin. Pattering of fin segmentation in A) wildtype, B) alf and C) sof pectoral fins; stacked blue rectangles model sequential addition and segmentation of individual rays. D) Schematic of regenerating pectoral fin assay to assess relative scaling. One pectoral fin is cut to approximately 50% of original size and allowed to regenerate. Comparisons to the contralateral side enables analysis of previous size and effect of treatment on non-regenerating fins. E) Dose response of FK506 treatment of pectoral fin growth. F-H) Pattern of segmentation of the lepidotrichia in F) regenerating wild-type, or regenerating FK506 treated wild-type or sof fins; solid line, plane of resection. While segments are normally restored during regeneration, regenerating fins treated with FK506 show elongated segmentation after regeneration similar to the alf phenotype (B). Error bars represent ± SEM. * p-value < 0.05. *** p-value < 0.001.