FIGURE
Fig. 4
- ID
- ZDB-FIG-180620-70
- Publication
- Minegishi et al., 2018 - Mutation in the Zebrafish cct2 Gene Leads to Abnormalities of Cell Cycle and Cell Death in the Retina: A Model of CCT2-Related Leber Congenital Amaurosis
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Fig. 4
Rescue of a small eye phenotype by CCT2 RNA injection. (A) Representative pictures of 2 dpf embryos after injection of wild-type human CCT2 RNA (100 pg). Genotyping of injected embryos is shown below. Ref. panel shows uninjected cct2-L394H-7del mutant. (B) Western blot analysis of indicated proteins in 2-dpf embryos after injection of RNA encoding wild-type or LCA-causing T400P CCTβ. Normal and mutant lines correspond to uninjected control and mutant larvae. Numbers below each lane indicate changes in the protein level relatively to uninjected normal larvae after normalization using Poncer S (Pon. S) staining. Two independent sets of embryos were analyzed. (C) Western blot analysis of indicated proteins in 3-dpf embryos. Three independent sets of embryos were analyzed. (D–E) The zn-5 staining of homozygous 3-dpf cct2-L394H-7del mutant eye section after mocked (D) or wild-type CCT2 RNA (E) injection. (F) The zn-5 staining of 3-dpf wild-type eye section after wild-type CCT2 RNA injection. Size-rescued mutant retinas demonstrated zn-5 staining and lamination comparable to those of wild-type retina. (G–I) The TUNEL staining of the eye sections as in (D–F). Note the absence of TUNEL-positive cells in the size-rescued mutant retina.
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Full text @ Invest. Ophthalmol. Vis. Sci.