ZFIN Figure: Lopez-Baez et al., 2018, Fig. 1-S2

ZFIN ID: ZDB-FIG-180613-7

ZFIN ID: ZDB-FIG-180613-7

Lopez-Baez et al., 2018 - Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.. eLIFE 7 Full text @ Elife

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibody:	Ab4-wt1
Fish:	mitfa^w2/+; mpv17^a9/+; li2Tg/+
Condition:	perforation: notochord
Anatomical Terms:	kidney regenerating tissue notochord outer sheath cell
Stage:	Day 6

Fig. 1-S2

Wt1 and GFP protein expression in the notochord.

(a) Immunohistochemistry of injured and uninjured notochord regions, stained with α-GFP and α-Wt1 antibodies. Sheath cells and vacuolated cell populations, and injury site are indicated. n > 10; five experimental replicates. (b) Immunofluorescence of 3 dpi injured notochord tissue stained with α-Wt1 antibody. Kidney tissue is shown as a positive control. dpi: days post injury. Experimental replicates = 5; n = 5.

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Qualifier	Anatomy
Ab4-wt1	IHC	mitfa^w2/+; mpv17^a9/+; li2Tg/+	standard conditions	Day 6	Not Detected	notochord
	IHC	mitfa^w2/+; mpv17^a9/+; li2Tg/+	perforation: notochord	Day 6		kidney
	IHC			Day 6		regenerating tissue notochord outer sheath cell

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife