|ZFIN ID: ZDB-FIG-180613-7|
Wt1 and GFP protein expression in the notochord.
(a) Immunohistochemistry of injured and uninjured notochord regions, stained with α-GFP and α-Wt1 antibodies. Sheath cells and vacuolated cell populations, and injury site are indicated. n > 10; five experimental replicates. (b) Immunofluorescence of 3 dpi injured notochord tissue stained with α-Wt1 antibody. Kidney tissue is shown as a positive control. dpi: days post injury. Experimental replicates = 5; n = 5.
|Ab4-wt1||IHC||mitfaw2/+; mpv17a9/+; li2Tg/+||standard conditions||Day 6||Not Detected||notochord|
|IHC||mitfaw2/+; mpv17a9/+; li2Tg/+||perforation: notochord||Day 6||kidney|
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