ZFIN ID: ZDB-FIG-180613-7
Lopez-Baez et al., 2018 - Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Day 6

Fig. 1-S2

Wt1 and GFP protein expression in the notochord.

(a) Immunohistochemistry of injured and uninjured notochord regions, stained with α-GFP and α-Wt1 antibodies. Sheath cells and vacuolated cell populations, and injury site are indicated. n > 10; five experimental replicates. (b) Immunofluorescence of 3 dpi injured notochord tissue stained with α-Wt1 antibody. Kidney tissue is shown as a positive control. dpi: days post injury. Experimental replicates = 5; n = 5.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab4-wt1 IHC mitfaw2/+; mpv17a9/+; li2Tg/+ standard conditions Day 6 Not Detected notochord
IHC mitfaw2/+; mpv17a9/+; li2Tg/+ perforation: notochord Day 6 kidney
IHC Day 6 regenerating tissue notochord outer sheath cell
Phenotype Details No data available
Acknowledgments:
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