ZFIN Figure: Lopez-Baez et al., 2018, Fig. 6

ZFIN ID: ZDB-FIG-180613-14

ZFIN ID: ZDB-FIG-180613-14

Lopez-Baez et al., 2018 - Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.. eLIFE 7 Full text @ Elife

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	EGFP KillerRed
Fish:	mitfa^w2/+; mpv17^a9/+; hu7478Tg; li2Tg/+
Condition:	perforation: notochord
Anatomical Term:	notochord regenerating tissue
Stage Range:	Protruding-mouth to Day 6

Fig. 6

Distinct and closely associated wt1b and entpd5a subpopulations emerge at the damage site.

(a) Live-imaging at the site of notochord injury in Tg(wt1b:gfp; entpd5a:dkRed) larvae. Expression of wt1b:gfp and entpd5a:pkRed at site of damage (green arrows and red arrows respectively) in injured and uninjured fish. n > 10; experimental replicates = 5. Scale bar: 50 µm. (b) Cryo-section of the injured area confirms distinct wt1b:gfp and entpd5a:dkRed subpopulations at site of damage. n > 10; experimental replicates = 2. Scale bar: 20 µm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
EGFP	mitfa^w2/+; mpv17^a9/+; hu7478Tg; li2Tg/+	standard conditions	Protruding-mouth	Not Detected	notochord	IFL
			Day 4	Not Detected	notochord	IFL
			Day 6	Not Detected	notochord	IFL
	mitfa^w2/+; mpv17^a9/+; hu7478Tg; li2Tg/+	perforation: notochord	Protruding-mouth		notochord regenerating tissue	IFL
			Day 4		notochord regenerating tissue	IFL
			Day 6		notochord regenerating tissue	IHC
			Day 6		notochord regenerating tissue	IFL
KillerRed	mitfa^w2/+; mpv17^a9/+; hu7478Tg; li2Tg/+	standard conditions	Protruding-mouth	Not Detected	notochord	IFL
			Day 4	Not Detected	notochord	IFL
			Day 6	Not Detected	notochord	IFL
	mitfa^w2/+; mpv17^a9/+; hu7478Tg; li2Tg/+	perforation: notochord	Protruding-mouth	Not Detected	notochord	IFL
			Day 4		notochord regenerating tissue	IFL
			Day 6		notochord regenerating tissue	IHC
			Day 6		notochord regenerating tissue	IFL

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife