ZFIN ID: ZDB-FIG-180613-14
Lopez-Baez et al., 2018 - Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: Protruding-mouth to Day 6

Fig. 6

Distinct and closely associated wt1b and entpd5a subpopulations emerge at the damage site.

(a) Live-imaging at the site of notochord injury in Tg(wt1b:gfp; entpd5a:dkRed) larvae. Expression of wt1b:gfp and entpd5a:pkRed at site of damage (green arrows and red arrows respectively) in injured and uninjured fish. n > 10; experimental replicates = 5. Scale bar: 50 µm. (b) Cryo-section of the injured area confirms distinct wt1b:gfp and entpd5a:dkRed subpopulations at site of damage. n > 10; experimental replicates = 2. Scale bar: 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ standard conditions Protruding-mouth Not Detected notochord IFL
Day 4 Not Detected notochord IFL
Day 6 Not Detected notochord IFL
mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ perforation: notochord Protruding-mouth notochord regenerating tissue IFL
Day 4 notochord regenerating tissue IFL
Day 6 notochord regenerating tissue IHC
Day 6 notochord regenerating tissue IFL
KillerRed mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ standard conditions Protruding-mouth Not Detected notochord IFL
Day 4 Not Detected notochord IFL
Day 6 Not Detected notochord IFL
mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ perforation: notochord Protruding-mouth Not Detected notochord IFL
Day 4 notochord regenerating tissue IFL
Day 6 notochord regenerating tissue IHC
Day 6 notochord regenerating tissue IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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