ZFIN ID: ZDB-FIG-180613-14 |
Lopez-Baez et al., 2018
- Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair..
eLIFE
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Full text @ Elife
EXPRESSION / LABELING:
Genes: | |
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Fish: | |
Condition: | |
Anatomical Term: | |
Stage Range: | Protruding-mouth to Day 6 |
Fig. 6 Distinct and closely associated wt1b and entpd5a subpopulations emerge at the damage site. (a) Live-imaging at the site of notochord injury in Tg(wt1b:gfp; entpd5a:dkRed) larvae. Expression of wt1b:gfp and entpd5a:pkRed at site of damage (green arrows and red arrows respectively) in injured and uninjured fish. n > 10; experimental replicates = 5. Scale bar: 50 µm. (b) Cryo-section of the injured area confirms distinct wt1b:gfp and entpd5a:dkRed subpopulations at site of damage. n > 10; experimental replicates = 2. Scale bar: 20 µm. |
Gene Expression Details
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
---|---|---|---|---|---|---|---|
EGFP | mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ | standard conditions | Protruding-mouth | Not Detected | notochord | IFL | |
Day 4 | Not Detected | notochord | IFL | ||||
Day 6 | Not Detected | notochord | IFL | ||||
mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ | perforation: notochord | Protruding-mouth | notochord regenerating tissue | IFL | |||
Day 4 | notochord regenerating tissue | IFL | |||||
Day 6 | notochord regenerating tissue | IHC | |||||
Day 6 | notochord regenerating tissue | IFL | |||||
KillerRed | mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ | standard conditions | Protruding-mouth | Not Detected | notochord | IFL | |
Day 4 | Not Detected | notochord | IFL | ||||
Day 6 | Not Detected | notochord | IFL | ||||
mitfaw2/+; mpv17a9/+; hu7478Tg; li2Tg/+ | perforation: notochord | Protruding-mouth | Not Detected | notochord | IFL | ||
Day 4 | notochord regenerating tissue | IFL | |||||
Day 6 | notochord regenerating tissue | IHC | |||||
Day 6 | notochord regenerating tissue | IFL |
Antibody Labeling Details
No data available
Phenotype Details
No data available
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife |
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