FIGURE

Fig. 5

ID

ZDB-FIG-180517-6

Publication

Zhang et al., 2017 - Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

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Fig. 5

The effects of leucine treatment on autophagy in zebrafish testis. (A) The mRNA expression of autophagy related genes atg5, atg7, atg12 and p62 were evaluated by qRT-PCR. (B) Representative immunoblots showed P62 and Lc3 proteins after leucine and LY294002 administration. Actin was used as the control. Relative protein levels of P62 and Lc3-II were shown from 3 independent experiments. (C) Representative immunofluorescence pictures to detect Lc3 levels in control and leucine-treated zebrafish testes. Scale bar: 50 μm. Bars represent mean ± SEM of more than three separate experiments. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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