Fig. 1

Rad21 and CTCF are present pre-ZGA and can be effectively depleted in early zebrafish development. (A) As embryos reach MZT, maternal transcripts are degraded and zygotic transcripts accumulate. The series of embryos below represents time points that were sampled for RNA-seq. hpf, hours post-fertilization. (B) Transcript numbers expressed as fragments per kilobase mapped (FPKM) of Rad21 and CTCF, measured by RNA-seq across the indicated time points. Error bars represent 95% confidence intervals. (C) Quantitation of immunoblots for Rad21 and CTCF protein levels, normalized against those of γ -tubulin. Data are mean±s.d. n=3. (D) Quantitation of immunoblots for Rad21 and CTCF protein levels, following depletion of these proteins using morpholino oligonucleotides [Rad21 knockdown (KD) and CTCF KD]. Protein levels are expressed as a percentage of wild-type levels and were normalized against those of γ -tubulin. Images of all immunoblots are provided in Fig. S1E.