FIGURE

Fig. s4

ID
ZDB-FIG-180404-15
Publication
Lagendijk et al., 2017 - Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
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Fig. s4

Acute loss of blood flow does not inflict immediate changes in tension across VE-cadherin (a) Junctional morphology of ECs in the DA (Venus, grey) of an uic and a sih MO injected embryo at 2 dpf showing that mature junctions fail to form without flow. Scale bar = 10μm. (b) Morphology of junctions in the DA (Venus, grey) of a control embryo and an embryo that has been incubated in a high dose of Tricaine (800mg/ml) to stop cardiac contraction and blood flow. Scale bar = 10μm. (c) Ratio-metric FRET values in junctions of control (n=68 junctional ROIs from n=10 embryos) versus Tricaine treated embryos control (n=56 junctional ROIs from n=10 embryos). Error bars represent mean ± s.d.; not significant (ns) p=0.0834, from unpaired two-sided ttest. (d) Venus expression (grey) showing junctional morphology of ECs in the DA at 3 dpf of a control embryo and an embryo that underwent surgical outflow tract resection to stop blood flow. Image taken 3 hours after surgery. Scale bar = 10μm. (e) Ratio-metric FRET quantifications from control embryos (n=39 junctional ROIs from n=8 embryos) and embryos in which the outflow tract was surgically disconnected (n=38 junctional ROIs from n=7 embryos). Error bars represent mean ± s.d.; not significant (ns) p=0.01472, from unpaired two-sided t-test. (f) Morphology of junctions in the DA (Venus, grey) of an untreated control versus a 2,3- butanedione monoxime (BDM, 50mM) treated embryo at 2 dpf. Scale bar = 10μm. (g) Ratio-metric FRET quantifications from junctions in untreated embryos (n=28 junctional ROIs from n=8 embryos) versus junctions in BDM (50mM) treated embryos (n=34 junctional ROIs from n=8 embryos) at 2 dpf. Error bars represent mean ± s.d.; not significant (ns) p=0.1039, from unpaired two-sided t-test.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.